TA502760

antibody from OriGene

Targeting: DNAJB2 CMT2T, HSJ1, HSPF3

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

TA502760

Provider

OriGene

Proper citation

OriGene Cat#TA502760, RRID:AB_11126483

Product name

DNAJB2 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Antibody type

Monoclonal

Description

DNAJB2 mouse monoclonal antibody, clone OTI4D12 (formerly 4D12)

Host

Mouse

Conjugate

Unconjugated

Epitope

DNAJB2

Isotype

IgG

Antibody clone number

OTI4D12

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DNAJB2 (RC206769, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-DNAJB2.

Validation comment

WB

Immunocytochemistry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Anti-DNAJB2 mouse monoclonal antibody (TA502760) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY DNAJB2(RC206769).

Validation comment

IF

Flow cytometry

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells transfected with either RC206769 overexpress plasmid(Red) or empty vector control plasmid(Blue) were immunostained by anti-DNAJB2 antibody(TA502760), and then analyzed by flow cytometry.

Validation comment

FC

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Flow cytometric Analysis of Jurkat cells, using anti-DNAJB2 antibody(TA502760),(Red), compared to a nonspecific negative control antibody,(Blue).

Validation comment

FC