TA500013

antibody from OriGene

Targeting: FGF2 FGFB

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

TA500013

Provider

OriGene

Proper citation

OriGene Cat#TA500013, RRID:AB_2104313

Product name

bFGF (FGF2) mouse monoclonal antibody, clone OTI2H11 (formerly 2H11)

Antibody type

Monoclonal

Description

bFGF (FGF2) mouse monoclonal antibody, clone OTI2H11 (formerly 2H11)

Host

Mouse

Conjugate

Unconjugated

Epitope

FGF2

Isotype

IgG

Antibody clone number

OTI2H11

Vial size

100 µl

Concentration

1.00mg/ml

Western blot

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-BFGF monoclonal antibody.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

BFGF antibody (2H11) at 1:10000 + Recombinant human bFGF (Cat# RC217426)

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY BFGF (RC217426, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-BFGF.

Validation comment

WB

Supportive validation

Submitted by

OriGene (provider)

Main image

Experimental details

Western blot analysis of extracts (10ug) from a mouse cell line and 3 different mouse tissues by using anti-BFGF monoclonal antibody.(1:200)

Validation comment

WB