Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- ELISA [1]
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- Product number
- DP3506S - Provider product page
- Provider
- Acris Antibodies GmbH
- Proper citation
- Acris Antibodies GmbH Cat#DP3506S, RRID:AB_981304
- Product name
- anti FGF basic / FGF2
- Antibody type
- Polyclonal
- Antigen
- Highly pure (> 98%) recombinant Human FGF-2 (basic) [Ala136 â Ser288] produced in E.coli (Cat.-No DA3503XC).
- Reactivity
- Human
- Host
- Rabbit
- Vial size
- 0.1 mg
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Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Test of cross-reactivity of anti-Human FGF-2 polyclonal antibody by Western blot analysis. Recombinant human, mouse and Rat FGF-2 produced in E. coli were loaded under reducing conditions in 15% SDS-polyacrylamide gel. Anti-human FGF-2 recognizes also the mouse and the rat form.
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- Western analysis of recombinant human (DA3503XC), mouse (AR31056PU-L) and rat (AR31055PU-L) FGF-2 (basic) using a polyclonal antibody directed against recombinant human FGF-2. As expected the antibody cross reacts with the mouse and rat proteins.
Supportive validation
- Submitted by
- Acris Antibodies GmbH (provider)
- Main image
- Experimental details
- FGF-2 Sandwich-ELISA using a polyclonal Goat anti-human FGF-2 as capture antibody and recombinant human FGF-2 (Cat.-No DA3503X) as standard. The polyclonal rabbit anti-human FGF-2 antibody (Cat.-No DP3506)in combination with a goat anti-rabbit-Biotin was used for detection.