PA5-78224

Semaniakou A, Chappe F, Anini Y, Chappe V
Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society 2021 Sep;20(5):881-890

Deletion of AMPA receptor GluA1 subunit gene (Gria1) causes circadian rhythm disruption and aberrant responses to environmental cues.

Ang G, Brown LA, Tam SKE, Davies KE, Foster RG, Harrison PJ, Sprengel R, Vyazovskiy VV, Oliver PL, Bannerman DM, Peirson SN
Translational psychiatry 2021 Nov 15;11(1):588

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Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of VIP in whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with VIP polyclonal antibody (Product # PA5-78224) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western blot analysis of VIP in whole cell lysate using 30 µg of protein. Samples were separated with 15% SDS-PAGE and incubated with VIP polyclonal antibody (Product # PA5-78224) using a dilution of 1:1000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Western Blot using VIP Polyclonal Antibody (Product # PA5-78224). Various whole cell extracts (30 µg) were separated by 15% SDS-PAGE, and the membrane was blotted with VIP Polyclonal Antibody (Product # PA5-78224) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: VIP Polyclonal Antibody detects VIP protein by western blot analysis. Whole cell extracts (30 µg) was separated by 15% SDS-PAGE, and the membrane was blotted with VIP Polyclonal Antibody (Product # PA5-78224) at a dilution of 1:1,000.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunocytochemistry-Immunofluorescence analysis of VIP was performed in DIV9 rat E18 primary hippocampal neuron cells fixed in 4% paraformaldehyde at RT for 15 min. Green: VIP Polyclonal Antibody (Product # PA5-78224) diluted at 1:500. Red: beta Tubulin 3/ Tuj1, stained by beta Tubulin 3/ Tuj1 antibody. Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) analysis of VIP was performed in frozen-sectioned mouse thalamus tissue using VIP Polyclonal Antibody (Product # PA5-78224) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: VIP Polyclonal Antibody detects VIP protein at cytosol on mouse pancreas by immunohistochemical analysis. Sample: Paraffin-embedded mouse pancreas. VIP Polyclonal Antibody (Product # PA5-78224) dilution: 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry (Frozen) analysis of VIP was performed in frozen-sectioned mouse thalamus tissue using VIP Polyclonal Antibody (Product # PA5-78224) at a dilution of 1:250 (Green). Blue: Fluoroshield with DAPI.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Fig. 4 Immunofluorescence cFos, VIP, and AVP signals. a A schematic depicting the timeline of the experiment: For half of the animals ( n = 3 wild-type and three GluA1 KO mice), no light pulse was given prior to perfusion and they were perfused at ~ZT15.5 (day 1, LP -); the remaining animals perfused on day 2 ( n = 3 wild-type and three GluA1 KO mice) received a nocturnal light pulse ( LP +) which started at ZT14. Star symbols indicate the Zeitgeber time of perfusion, which was ~90 min after the onset of the nocturnal light (~ZT15.5). Thus, the ZTs at which perfusion was performed were matched between LP - and LP + conditions. b Representative images of immunofluorescence cFos, VIP, and AVP staining in the SCN (scale bar = 100 mum). In the middle multi-panels, brighter colours indicate higher immunofluorescence ( F ) intensity, whereas darker colours indicate lower F intensity. A schematic delineating the core (ventromedial) vs. shell (dorsolateral) subregions of the SCN is shown on the right (D dorsal, V ventral, M medial, and L lateral). c Mean F intensities (+-standard errors of the mean) from all three channels across the entire mediolateral extent of the SCN. Nocturnal-light-induced cFos signals were attenuated in GluA1 knockouts (*two-way ANOVA for LP + conditions, Genotype x Mediolateral Extent interaction p = 0.019). VIP and AVP data were pooled across LP - and + LP ( n = 6 per genotype). The vertical dashed lines represent the location of the midline, and the 60-mum