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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [3]
- Immunohistochemistry [3]
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Validation data
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- Product number
- GTX84246 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX84246, RRID:AB_10730217
- Product name
- Cytokeratin 8 antibody [1B12]
- Antibody type
- Monoclonal
- Reactivity
- Human
- Host
- Mouse
- Storage
- For short-term storage, store at 4¢XC or aliquot into working amounts and store at -20¢XC. For long-term storage, store at -70¢XC (aliquotted). Avoid repeated freeze-thaw cycles.
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Supportive validation
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- GeneTex (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (GTX84246).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (GTX84246).
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Anti-KRT8 mouse monoclonal antibody (GTX84246) immunofluorescent staining of COS7 cells transiently transfected with KRT8
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human colon tissue using anti-KRT8 mouse monoclonal antibody. (GTX84246)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human ovary tissue using anti-KRT8 mouse monoclonal antibody. (GTX84246)
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded human breast tissue using anti-KRT8 mouse monoclonal antibody. (GTX84246)
