Explore
Validate
Learn
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- TA500020 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500020, RRID:AB_1528120
- Product name
- CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)
- Antibody type
- Monoclonal
- Description
- CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B9 (formerly 1B9)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRT8
- Isotype
- IgG
- Antibody clone number
- OTI1B9
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references M1 macrophage mediated increased reactive oxygen species (ROS) influence wound healing via the MAPK signaling in vitro and in vivo.
Deng Z, Shi F, Zhou Z, Sun F, Sun MH, Sun Q, Chen L, Li D, Jiang CY, Zhao RZ, Cui D, Wang XJ, Jing YF, Xia SJ, Han BM
Toxicology and applied pharmacology 2019 Mar 1;366:83-95
Toxicology and applied pharmacology 2019 Mar 1;366:83-95
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-KRT8 mouse monoclonal antibody (TA500020) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
- Validation comment
- IF
