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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [6]
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- Product number
- TA500021 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500021, RRID:AB_2134838
- Product name
- CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)
- Antibody type
- Monoclonal
- Description
- CK8 (Keratin 8) mouse monoclonal antibody, clone OTI1B12 (formerly 1B12)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- KRT8
- Isotype
- IgG
- Antibody clone number
- OTI1B12
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Stress chaperone mortalin contributes to epithelial-mesenchymal transition and cancer metastasis.
Na Y, Kaul SC, Ryu J, Lee JS, Ahn HM, Kaul Z, Kalra RS, Li L, Widodo N, Yun CO, Wadhwa R
Cancer research 2016 May;76(9):2754-2765
Cancer research 2016 May;76(9):2754-2765
No comments: Submit comment
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-KRT8 monoclonal antibody.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY KRT8 (RC209570, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-KRT8.
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (10ug) from 8 Human tissue by using anti-KRT8 monoclonal antibody at 1:1000 (1: Testis; 2: Uterus; 3: Breast; 4: Brain; 5: Liver; 6: Ovary; 7: Thyroid gland; 8: Colon).
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-KRT8 mouse monoclonal antibody (TA500021).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-KRT8 mouse monoclonal antibody (TA500021) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY KRT8(RC209570).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of A549 cells using anti-KRT8 mouse monoclonal antibody (TA500021).
- Validation comment
- IF
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human ovary tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human prostate tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-KRT8 mouse monoclonal antibody (TA500021) at 1:150 dilution.
- Validation comment
- IHC
