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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [2]
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- Product number
- PA5-28985 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Cytokeratin 8 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji, mouse liver. Predicted reactivity: Mouse (87%), Rat (88%), Dog (81%), Pig (85%), Rhesus Monkey (98%), Bovine (87%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.53 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Quantitative interactome analysis reveals a chemoresistant edgotype.
Chavez JD, Schweppe DK, Eng JK, Zheng C, Taipale A, Zhang Y, Takara K, Bruce JE
Nature communications 2015 Aug 3;6:7928
Nature communications 2015 Aug 3;6:7928
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 8 Polyclonal Antibody (Product # PA5-28985). Sample (30 µg whole cell lysate). A: MOLT4. B: Raji. 10% SDS PAGE. Cytokeratin 8 Polyclonal Antibody (Product # PA5-28985) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Cytokeratin 8 Polyclonal Antibody (Product # PA5-28985). Sample (50 µg of whole cell lysate). Lane A: mouse liver. 10% SDS PAGE. Cytokeratin 8 Polyclonal Antibody (Product # PA5-28985) diluted at 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of Cytokeratin 8 in paraformaldehyde-fixed HeLa cells using a Cytokeratin 8 polyclonal antibody (Product # PA5-28985) at a 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of Cytokeratin 8 was performed in paraffin-embedded mouse duodenum tissue using Cytokeratin 8 Polyclonal Antibody (Product # PA5-28985) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded A549 xenograft, using Cytokeratin 8 (Product # PA5-28985) antibody at 1:500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
