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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
- Immunoprecipitation [1]
- Immunohistochemistry [1]
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- Product number
- H00003431-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00003431-M01, RRID:AB_715088
- Product name
- Mouse Monoclonal SP110 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified. SP110 (8C8)
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP110 Antibody (8C8) [H00003431-M01] - SP110 expression in Jurkat ( Cat # L017V1 ).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi ( Cat # H00003431-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody (M01), clone 8C8 (Cat # H00003431-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody (M01), clone 8C8.Lane 1: SP110 transfected lysate(61.8 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- ELISA: SP110 Antibody (8C8) [H00003431-M01] - Detection limit for recombinant GST tagged SP110 is approximately 0.1ng/ml as a capture antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SP110 Antibody (8C8) [H00003431-M01] - Analysis of SP110 transfected lysate using anti-SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 MaxPab rabbit polyclonal antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: SP110 Antibody (8C8) [H00003431-M01] - Analysis of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.
