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Western blot
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- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- PAB9928 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB9928, RRID:AB_1671612
- Product name
- ADAM10 polyclonal antibody
- Antibody type
- Polyclonal
- Antigen
- A synthetic peptide corresponding to C-terminus of human ADAM10.
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Vial size
- 100 μg
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Processing of the notch ligand delta by the metalloprotease Kuzbanian.
Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases.
Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis.
Qi H, Rand MD, Wu X, Sestan N, Wang W, Rakic P, Xu T, Artavanis-Tsakonas S
Science (New York, N.Y.) 1999 Jan 1;283(5398):91-4
Science (New York, N.Y.) 1999 Jan 1;283(5398):91-4
Identification and characterization of a pro-tumor necrosis factor-alpha-processing enzyme from the ADAM family of zinc metalloproteases.
Rosendahl MS, Ko SC, Long DL, Brewer MT, Rosenzweig B, Hedl E, Anderson L, Pyle SM, Moreland J, Meyers MA, Kohno T, Lyons D, Lichenstein HS
The Journal of biological chemistry 1997 Sep 26;272(39):24588-93
The Journal of biological chemistry 1997 Sep 26;272(39):24588-93
Kuzbanian controls proteolytic processing of Notch and mediates lateral inhibition during Drosophila and vertebrate neurogenesis.
Pan D, Rubin GM
Cell 1997 Jul 25;90(2):271-80
Cell 1997 Jul 25;90(2):271-80
No comments: Submit comment
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot using ADAM10 polyclonal antibody (Cat # PAB9928) shows detection of ADAM10 in Jurkat whole cell lysate in the absence (1) or presence (2) of blocking peptide.The membrane was probed with the primary antibody diluted to 1 : 1,000.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunocytochemistry using ADAM10 polyclonal antibody (Cat # PAB9928) shows staining of ADAM10 in K-562 cells. The cells were fixed with para-formaldehyde and reacted with primary antibody diluted to 1 : 500.
- Validation comment
- Immunocytochemistry
