PA1-12500

antibody from Invitrogen Antibodies

Targeting: ADAM10 CD156C, HsT18717, kuz, MADM

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA1-12500

Provider

Invitrogen Antibodies

Product name

ADAM10 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is expected to cross-react with bovine samples due to sequence homology.

Concentration

1 mg/mL

References

Submitted references

Loss of Adam10 Disrupts Ion Transport in Immortalized Kidney Collecting Duct Cells.

Assmus A, Mullins L, Ward M, Dobie R, Hunter R, Henderson NC, Mullins JJ
Function (Oxford, England) 2021;2(4):zqab024

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Behavioural effects and regulation of PKCalpha and MAPK by huprine X in middle aged mice.

Ratia M, Giménez-Llort L, Camps P, Muñoz-Torrero D, Clos MV, Badia A
Pharmacology, biochemistry, and behavior 2010 Jun;95(4):485-93

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of ADAM10 using a polyclonal antibody (Product # PA1-12500).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 3. Identification of 2 Clones Resulting From Targeting of the Adam10 Gene in mCCD cl1 . (A) Transfection results on mCCD cl1 cell population for the Adam10 gene. ""Del"" for deletion; WT represents the predicted size of the WT product. T7 product of G2 is a doublet. (B) T7 screening for Adam10 transfected mCCD cl1 cells populations, following single-cell cloning. Red arrows indicate the clones selected after screening, based on the disappearance of a WT-sized band. ""Ins"" for insertion; ""Del"" for deletion; WT represents the predicted size of the WT product. The numbers above each gel track are the initial identification numbers of each clone. (C) Sequencing of PCR products from both clonal cell lines for Adam10. (D) Immunostaining of mCCD cl1 cells using anti-Adam10 antibody in wild-type mCCD cl1 cells (WT) and KO clonal lines A1 and A5. Composite image with DAPI staining of cell nuclei (blue). All scale bars 30 mum.