Explore
Validate
Learn
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunocytochemistry [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- BAF5664 - Provider product page
- Provider
- R&D Systems
- Product name
- Human Syntaxin 6 Biotinylated Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Syntaxin 6 antibodies are ideal for immunocytochemistry colocalization studies in trans-Golgi and endosomal membranes. The unconjugated antibody detects human Syntaxin 6 in direct ELISAs and Western blots. In direct ELISAs, less than 3% cross-reactivity with recombinant human (rh) Syntaxin 8, rhSyntaxin 12, and rhSyntaxin 16 is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Biotin
- Antigen sequence
O43752- Isotype
- IgG
- Vial size
- 50 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
No comments: Submit comment
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Syntaxin 6 in HeLa Human Cell Line. Syntaxin 6 was detected in formaldehyde fixed HeLa human cervical epithelial carcinoma cell line using Sheep Anti-Human Syntaxin 6 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF5664) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to Golgi bodies. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
