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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- ARG51847 - Provider product page
- Provider
- Arigo
- Product name
- anti-Niban phospho (Ser602) antibody
- Antibody type
- Polyclonal
- Antigen
- Peptide sequence around phosphorylation site of serine 602 (R-A-S(p)-A-I) derived from Human Niban.
- Description
- Antibodies were produced by immunizing rabbits with KLH-conjugated synthetic phosphopeptide. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. In addition, non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µl
- Concentration
- 1 mg/ml
- Storage
- Aliquot and store at -20°C. Storage in frost free freezers is not recommended. Avoid repeated freeze/thaw cycles. The antibody solution should be gently mixed before use.
- Handling
- The antibody solution should be gently mixed before use.
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Supportive validation
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- Arigo (provider)
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- Experimental details
- Western blot: in vitro kinase assays were performed by mixing purified bacterially expressed WT His-Niban or the His-Niban S602A with or without purified active AKT1 stained with anti-Niban (phospho Ser602) antibody ARG51847.
