21333-1-AP

antibody from Invitrogen Antibodies

Targeting: NIBAN1 C1orf24, FAM129A, GIG39, NIBAN

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

21333-1-AP

Provider

Invitrogen Antibodies

Product name

FAM129A Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: MGGSASSQL DEGKCAYIRG KTEAAIKNFS PYYSRQYSVA FCNHVRTEVE QQRDLTSQFL KTKPPLAPGT ILYEAELSQF SEDIKKWKER YVVVKNDYAV ESYENKEAYQ RGAAPKCRIL PAGGKVLTSE DEYNLLSDRH FPDPLASSEK ENTQPFVVLP KEFPVYLWQP FFRHGYFCFH EAADQKRFSA LLSDCVRHLN HDYMKQMTFE AQAFLEAVQF FRQEKGHYGS WEMITGDEIQ ILSNLVMEEL LPTLQTDLLP KMKGKKNDRK RTWLGLLEEA YTLVQHQVSE GLSALKEECR ALTKGLEGTI RSDMDQIVNS KNYLIGKIKA MVAQPAEKSC LESVQPFLAS I (1-350 aa encoded by BC030531)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.5 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

A431 cells were subjected to SDS PAGE followed by western blot with 21333-1-AP (FAM129A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

DU 145 cells were subjected to SDS PAGE followed by western blot with 21333-1-AP (FAM129A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

HT-1080 cells were subjected to SDS PAGE followed by western blot with 21333-1-AP (FAM129A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM129A antibody (Product # 21333-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of HepG2 cells, using FAM129A antibody 21333-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IGG (red).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of Hela cells, using FAM129A antibody 21333-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas slide using 21333-1-AP (FAM129A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human pancreas slide using 21333-1-AP (FAM129A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human thyroid cancer slide using 21333-1-AP (FAM129A Antibody) at dilution of 1:50.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human thyroid cancer slide using 21333-1-AP ( FAM129A Antibody) at dilution of 1:50.