Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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Validation data
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- Product number
- NBP2-67816 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Monoclonal Ubiquitin-activating Enzyme/UBE1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1:5000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Whole spinal cord extracts from P8 mice. UBE1 band can be seen at 130 kDa. Antibody at 1:500. Image from verified customer review.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Human skin fibroblast cell lysate, 5 ug total protein. Primary antibody at 1:500. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Flow Cytometry: Ubiquitin-activating Enzyme/UBE1 Antibody (SD08-62) [NBP2-67816] - Analysis of K562 cells with UBA1 antibody at 1:50 (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.