Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [2]
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Validation data
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- Product number
- 14883-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- KLHL12 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: NLLQYVRMP LLTPRYITDV IDAEPFIRCS LQCRDLVDEA KKFHLRPELR SQMQGPRTRA RLGANEVLLV VGGFGSQQSP IDVVEKYDPK TQEWSFLPSI TRKRRYVASV SLHDRIYVIG GYDGRSRLSS VECLDYTADE DGVWYSVAPM NVRRGLAGAT TLGDMIYVSG GFDGSRRHTS MERYDPNIDQ WSMLGDMQTA REGAGLVVAS GVIYCLGGYD GLNILNSVEK YDPHTGHWTN VTPMATKRSG AGVALLNDHI YVVGGFDGTA HLSSVEAYNI RTDSWTTVTS MTTPRCYVGA TVLRGRLYAI AGYDGNSLLS SIECYDPIID SWEVVTSMGT QRCDAGVCVL REK (217-568 aa encoded by BC004175)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.36 mg/mL
- Storage
- -20°C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 14883-1-AP (KLHL12 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 14883-1-AP (KLHL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of PC-3 cells using 14883-1-AP (KLHL12 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 10x lens).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry of paraffin-embedded human testis using 14883-1-AP (KLHL12 antibody) at dilution of 1:100 (under 40x lens).