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ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Flow cytometry [1]
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- Product number
- MAB10128-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human AdipoR1 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from cell culture supernatant. Detects human AdipoR1 in direct ELISAs. In direct ELISA, no cross-reactivity with human AdipoR2 is observed
- Reactivity
- Human
- Host
- Rabbit
- Conjugate
- Unconjugated
- Isotype
- IgG
- Antibody clone number
- 2416C
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- AdipoR1 in Human Placenta. AdipoR1 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in decidual cells. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of AdipoR1 in HEK293 Human Cell Line Transfected with Human AdipoR1 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human AdipoR1 or (B) irrelevant transfectants and eGFP was stained with Rabbit Anti-Human AdipoR1 Monoclonal Antibody (Catalog # MAB10128) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111). Quadrant markers were set based on control antibody staining (Catalog # MAB1050). View our protocol for Staining Membrane-associated Proteins.
