Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- PA5-21845 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- STK25 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji.
- Concentration
- 0.21 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of STK25 using 30 µg of HeLa S3 lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a STK25 polyclonal antibody (Product # PA5-21845) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
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- Experimental details
- Western Blot using STK25 Polyclonal Antibody (Product # PA5-21845). Various whole cell extracts (30 µg) were separated by 10% SDS-PAGE, and the membrane was blotted with STK25 Polyclonal Antibody (Product # PA5-21845) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of STK25 in methanol-fixed HeLa cells using a STK25 polyclonal antibody (Product # PA5-21845) at a 1:200 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using STK25 (Product # PA5-21845) antibody at 1:200 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using STK25 (Product # PA5-21845) antibody at 1:100 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.