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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- PAB10079 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB10079, RRID:AB_1679619
- Product name
- ING5 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Goat polyclonal antibody raised against synthetic peptide of ING5.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references p29ING4 and p28ING5 bind to p53 and p300, and enhance p53 activity.
Shiseki M, Nagashima M, Pedeux RM, Kitahama-Shiseki M, Miura K, Okamura S, Onogi H, Higashimoto Y, Appella E, Yokota J, Harris CC
Cancer research 2003 May 15;63(10):2373-8
Cancer research 2003 May 15;63(10):2373-8
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown using ING5 polyclonal antibody (Cat # PAB10079) to detect over expressed Human ING5 present in HeLa cell nuclear extracts.This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4on the same membrane (lane 2). A mock purification is shown inlane 1. Comparison to a molecular weight marker (not shown)indicates a single band of ~45.0 KDa corresponding to theexpected molecular weight for the recombinant protein. The blotwas incubated with a 1:500 dilution of the antibody at roomtemperature followed by detection using chemiluminescencereagent with a 5-min exposure time. Other detection systems willyield similar results. Personal communication Jacques Cote.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis is shown using ING5 polyclonal antibody (Cat # PAB10079) to detect with purified recombinant human ING5 protein.Indicates a single band of ~36 KDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ug of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose.The primary antibody diluted to 1 : 1,500.Followed by washes and reaction with a 1 : 20,000 dilution of IRDye™800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature.IRDye™800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.IRDye is a trademark of LI-COR, Inc.
