Explore
Validate
Learn
Western blot
ELISAAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
Submit
Validation data
Reference
Comment
Report error
- Product number
- GTX23716 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX23716, RRID:AB_380360
- Product name
- ING5 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human
- Host
- Goat
- Storage
- Store vial at -20¢X C prior to opening. Aliquot contents and freeze at -20¢X C or below for extended storage. Avoid cycles of freezing and thawing.
No comments: Submit comment
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis is shown using GeneTex Affinity Purified anti-p28 ING5 antibody (GTX23716) to detect over expressed Human ING5 present in HeLa cell nuclear extracts. This western blot shows reactivity with purified recombinant TAP tagged human ING5 protein (lane 3) and does not recognize TAP tagged ING4 on the same membrane (lane 2). A mock purification is shown in lane 1. Comparison to a molecular weight marker (not shown) indicates a single band of ~45.0 kDa corresponding to the expected molecular weight for the recombinant protein. The blot was incubated with a 1:500 dilution of the antibody at room temperature followed by detection using chemiluminescence reagent with a 5-min exposure time. Other detection systems will yield similar results.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Western blot analysis is shown using GeneTex's Affinity Purified anti-ING5 antibody to detect over expressed Human ING5 present in cell extracts. This western blot shows reactivity with purified recombinant human ING5 protein. Comparison to a molecular weight marker (not shown) indicates a single band of ~36 kDa corresponding to the expected molecular weight for the recombinant protein. Approximately 10 ?g of lysate was separated on a 4-20% Tris-Glycine gel by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1:1,500.. Incubation was overnight at 4¢X C followed by washes and reaction with a 1:20,000 dilution of IRDye?800 conjugated Rb-a-Goat IgG [H&L] MXHu for 45 min at room temperature. IRDye?800 fluorescence image was captured using the Odyssey? Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results.
