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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1204 - Provider product page
- Provider
- R&D Systems
- Product name
- Mouse IL-20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects mouse IL-20 in direct ELISAs and the Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-20 is observed.
- Reactivity
- Mouse
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
Q9JKV9- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-20 promotes epithelial healing of the injured mouse cornea.
Zhang W, Magadi S, Li Z, Smith CW, Burns AR
Experimental eye research 2017 Jan;154:22-29
Experimental eye research 2017 Jan;154:22-29
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Supportive validation
- Submitted by
- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-20 and Neutralization by Mouse IL-20 Antibody. Recombinant Mouse IL-20 (Catalog # 1204-ML) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 R alpha and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL-20 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1204). The ND50 is typically 0.2-0.6 µg/mL.
