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Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Blocking/Neutralizing [1]
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- Product number
- AF1102 - Provider product page
- Provider
- R&D Systems
- Product name
- Human IL-20 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Detects human IL-20 in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) IL-19 is observed, approximately 15% cross-reactivity with recombinant mouse IL-20 is observed, and less than 1% cross-reactivity with rhIL-10 is observed.
- Reactivity
- Human
- Host
- Goat
- Conjugate
- Unconjugated
- Antigen sequence
AAF36679- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references IL-20 Signaling in Activated Human Neutrophils Inhibits Neutrophil Migration and Function.
Induction of phenotype modifying cytokines by FERMT1 mutations.
The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
Gough P, Ganesan S, Datta SK
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 1;198(11):4373-4382
Journal of immunology (Baltimore, Md. : 1950) 2017 Jun 1;198(11):4373-4382
Induction of phenotype modifying cytokines by FERMT1 mutations.
Heinemann A, He Y, Zimina E, Boerries M, Busch H, Chmel N, Kurz T, Bruckner-Tuderman L, Has C
Human mutation 2011 Apr;32(4):397-406
Human mutation 2011 Apr;32(4):397-406
The Th17 cytokine IL-22 induces IL-20 production in keratinocytes: a novel immunological cascade with potential relevance in psoriasis.
Wolk K, Witte E, Warszawska K, Schulze-Tanzil G, Witte K, Philipp S, Kunz S, Döcke WD, Asadullah K, Volk HD, Sterry W, Sabat R
European journal of immunology 2009 Dec;39(12):3570-81
European journal of immunology 2009 Dec;39(12):3570-81
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Cell Proliferation Induced by IL-20 and Neutralization by Human IL-20 Antibody. Recombinant Human IL-20 (Catalog # 1102-IL) stimulates proliferation in the BaF3 mouse pro-B cell line co-transfected with human IL-20 R alpha and IL-20 R beta in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-20 (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-20 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1102). The ND50 is typically 0.1-0.4 µg/mL.
