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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- A300-271A - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- INT7 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- The recommended shelf life for this product is 1 year from date of receipt.
- Concentration
- 1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT7 by western blot. Sample: Nuclear extract (NE) from HeLa cells. Antibody: Rabbit anti-INT7 antibody (Cat. No. A300-271A) used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 15 minutes.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT7 by western blot. Samples: Whole cell lysate (50 µg) from HeLa and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-INT7 antibody A300-271A (lot A300-271A-1) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Detection of human INT7 by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- INT7 (Cat. No. A300-271A Lot1) used at a dilution of 1:1,000 (1µg/ml). Detection: DAB.
