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- Antibody Data
- Antigen structure
- References [1]
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- Western blot [1]
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- Product number
- AF7437 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BATF3 Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Detects human BATF3 in direct ELISAs and Western blots. In direct ELISAs, approximately 45% cross-reactivity with recombinant mouse BATF3 is observed, and less than 2% cross-reactivity with recombinant human BATF is observed.
- Reactivity
- Human
- Host
- Sheep
- Conjugate
- Unconjugated
- Antigen sequence
Q9NR55- Isotype
- IgG
- Vial size
- 100 ug
- Concentration
- LYOPH
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Type I interferon-enhanced IL-10 expression in human CD4 T cells is regulated by STAT3, STAT2, and BATF transcription factors.
Govender U, Corre B, Bourdache Y, Pellegrini S, Michel F
Journal of leukocyte biology 2017 May;101(5):1181-1190
Journal of leukocyte biology 2017 May;101(5):1181-1190
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of Human BATF3 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BATF3 at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- BATF3 in HDLM-2 Human Cell Line. BATF3 was detected in immersion fixed HDLM-2 human Hodgkin's lymphoma cell line using Sheep Anti-Human BATF3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7437) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and nuclei. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
