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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PAB1920 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB1920, RRID:AB_1579208
- Product name
- PROX1 polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of PROX1.
- Storage
- Store at 4°C. For long term storage store at -20°C.Aliquot to avoid repeated freezing and thawing.
Submitted references Mutations and aberrant DNA methylation of the PROX1 gene in hematologic malignancies.
Prox1 function controls progenitor cell proliferation and horizontal cell genesis in the mammalian retina.
Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate.
Nagai H, Li Y, Hatano S, Toshihito O, Yuge M, Ito E, Utsumi M, Saito H, Kinoshita T
Genes, chromosomes & cancer 2003 Sep;38(1):13-21
Genes, chromosomes & cancer 2003 Sep;38(1):13-21
Prox1 function controls progenitor cell proliferation and horizontal cell genesis in the mammalian retina.
Dyer MA, Livesey FJ, Cepko CL, Oliver G
Nature genetics 2003 May;34(1):53-8
Nature genetics 2003 May;34(1):53-8
Prox1 is a master control gene in the program specifying lymphatic endothelial cell fate.
Hong YK, Harvey N, Noh YH, Schacht V, Hirakawa S, Detmar M, Oliver G
Developmental dynamics : an official publication of the American Association of Anatomists 2002 Nov;225(3):351-7
Developmental dynamics : an official publication of the American Association of Anatomists 2002 Nov;225(3):351-7
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PROX1 polyclonal antibody (Cat # PAB1920) in HepG2 cell line lysates (35 ug/lane). PROX1 (arrow) was detected using the purified polyclonal antibody.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western blot analysis of PROX1 (arrow) using PROX1 polyclonal antibody (Cat # PAB1920). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the PROX1 gene (Lane 2) (Origene Technologies).
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Formalin-fixed and paraffin-embedded human hepatocellular carcinoma tissue reacted with PROX1 polyclonal antibody (Cat # PAB1920), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
- Validation comment
- Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
