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LearnPA5-69128
antibody from Invitrogen Antibodies
Targeting: RHOU
ARHU, CDC42L1, DJ646B12.2, fJ646B12.2, FLJ10616, hG28K, WRCH-1, WRCH1
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- PA5-69128 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- WRCH1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- This target displays homology in the following species: Cow: 100%; Dog: 100%; Guinea Pig: 79%; Horse: 100%; Human: 100%; Mouse: 93%; Pig: 100%; Rabbit: 100%; Rat: 79%
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
Submitted references The DNA-helicase HELLS drives ALK(-) ALCL proliferation by the transcriptional control of a cytokinesis-related program.
Tameni A, Sauta E, Mularoni V, Torricelli F, Manzotti G, Inghirami G, Bellazzi R, Fragliasso V, Ciarrocchi A
Cell death & disease 2021 Jan 27;12(1):130
Cell death & disease 2021 Jan 27;12(1):130
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of WRCH1 on human muscle tissue lysate. The sample was probed with a WRCH1 polyclonal antibody (Product # PA5-69128) using a primary antibody dilution of 0.2-1.0 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig. 5 Rho-GTPases mediate HELLS-downstream effects. A qRT-PCR analysis of RhoA, RhoU and Pak2 expression after single or combined siRNAs in TLBR-2 and MAC2A cell lines (36 h post-nucleofection). Each data represent mean +- SEM ( n = 3). Two-tailed t -test. * p < 0.05, ** p < 0.01. B Western blots show knockdown of RhoA, RhoU, or Pak2 36 h post-nucleofection with specific siRNAs in TLBR-2 and MAC2A. GAPDH was used as housekeeping gene. C The histograms show the percentage of multi-nucleated cells in at least 500 cells TLBR-2 and MAC2A stained with DAPI, F-actin, and beta-tubulin antibodies (48 h post-nucleofection). Cells were nucleofected with single and combined specific siRNAs against RhoA, RhoU, and Pak2. Each data point represents the mean +- SEM ( n = 3). Two-tailed t -test. * p < 0.05 and ** p < 0.01 relative to siRNA scramble. # p < 0.05 relative to RhoA KD , RhoU KD, or Pak2 KD . D The histograms show the percentage of cytokinetic cells in at least 500 cells TLBR-2 and MAC2A stained with DAPI, F-actin, and beta-tubulin antibodies (48 h post-nucleofection). The term ""abnormal"" refers to cytokinetic cells with defects in cleavage furrow and/or microtubules structures. Cells were nucleofected with single and combined specific siRNAs against RhoA and RhoU. Each data point represents the mean +- SEM ( n = 3). Two-tailed t -test. * p < 0.05 and ** p < 0.01 relative to siRNA scramble. E Immunofluorescence of TLBR-2 and MAC2A nucleofected with siRNA scramble and single and
