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Western blot
Blocking/NeutralizingAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- ELISA [1]
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- Product number
- H00005867-M01 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#H00005867-M01, RRID:AB_547528
- Product name
- Mouse Monoclonal Rab4 Antibody
- Antibody type
- Monoclonal
- Description
- IgG purified. RAB4A - RAB4A, member RAS oncogene family
- Reactivity
- Human
- Host
- Mouse
- Isotype
- IgG
- Vial size
- 0.1 mg
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - Analysis of RAB4A over-expressed 293 cell line, cotransfected with RAB4A Validated Chimera RNAi ( Cat # H00005867-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with RAB4A monoclonal antibody (M01), clone 1C10 (Cat # H00005867-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - Analysis of RAB4A expression in transfected 293T cell line by RAB4A monoclonal antibody (M01), clone 1C10.Lane 1: RAB4A transfected lysate(24.4 KDa).Lane 2: Non-transfected lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: Rab4 Antibody (1C10) [H00005867-M01] - RAB4A monoclonal antibody (M01), clone 1C10 Analysis of RAB4A expression in Hela.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Sandwich ELISA: Rab4 Antibody (1C10) [H00005867-M01] - Detection limit for recombinant GST tagged RAB4A is approximately 0.1ng/ml as a capture antibody.
