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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-G35 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-G35, RRID:AB_2610762
- Product name
- Anti-RAB4 (RABBIT) Antibody - 100-401-G35
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of rabbit anti-Rab4 antibody. Lane 1: HeLa cell lysate. Primary antibody: Rab4 antibody at 1:1000 for overnight at 4°C. Secondary antibody: Goat anti-rabbit IgG HRP secondary antibody at 1:10,000 for 45 min at RT. Block: 5% Blotto overnight 4°C. Predicted/Observed size:23.6 kDa/26kD. Other band(s): An additional protein of >100kDa is also detected. Additional cross-reactive bands are detected at ~75kDa and 50kDa in rat and mouse samples.
- Validation comment
- Western Blot
