Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [4]
- Immunocytochemistry [2]
- Immunohistochemistry [1]
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- Product number
- GTX109877 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX109877, RRID:AB_1950283
- Product name
- Fumarate hydratase antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Fumarase-deficient Uterine Leiomyomas: An Immunohistochemical, Molecular Genetic, and Clinicopathologic Study of 86 Cases.
Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Miettinen M, Felisiak-Golabek A, Wasag B, Chmara M, Wang Z, Butzow R, Lasota J
The American journal of surgical pathology 2016 Dec;40(12):1661-1669
The American journal of surgical pathology 2016 Dec;40(12):1661-1669
Mitochondrial proteomics with siRNA knockdown to reveal ACAT1 and MDH2 in the development of doxorubicin-resistant uterine cancer.
Lo YW, Lin ST, Chang SJ, Chan CH, Lyu KW, Chang JF, May EW, Lin DY, Chou HC, Chan HL
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
Journal of cellular and molecular medicine 2015 Apr;19(4):744-59
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Supportive validation
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- Fumarate hydratase antibody detects FH protein by western blot analysis. A. 50 ?g mouse liver lysate/extract 7.5% SDS-PAGE Fumarate hydratase antibody (GTX109877) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Experimental details
- Fumarate hydratase antibody detects Fumarate hydratase protein by western blot analysis.A.50 ?g rat liver lysate/extract 10% SDS-PAGEFumarate hydratase antibody (GTX109877) dilution: 1:5000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Sample (30 ?g of whole cell lysate)A: HeLaB: HepG2 (GTX27900)10% SDS PAGEGTX109877 diluted at 1:1000The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with Fumarate hydratase antibody (GTX109877) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
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- Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37¢J for 30 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.Blue: Hoechst 33342 staining.
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- Fumarate hydratase antibody detects Fumarate hydratase protein at mitochondria by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: Fumarate hydratase protein stained by Fumarate hydratase antibody (GTX109877) diluted at 1:500.Blue: Hoechst 33342 staining.
Supportive validation
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- Immunohistochemical analysis of paraffin-embedded NCIN87 xenograft, using fumarate hydratase (GTX109877) antibody at 1:500 dilution.