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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
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Validation data
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- Product number
- TA500681 - Provider product page
- Provider
- OriGene
- Proper citation
- OriGene Cat#TA500681, RRID:AB_2104091
- Product name
- FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)
- Antibody type
- Monoclonal
- Description
- FH (Fumarate Hydratase) mouse monoclonal antibody, clone OTI2A2 (formerly 2A2)
- Reactivity
- Canine
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- FH
- Isotype
- IgG
- Antibody clone number
- OTI2A2
- Vial size
- 100 µl
- Concentration
- 1.00mg/ml
Submitted references Fumarate hydratase loss promotes mitotic entry in the presence of DNA damage after ionising radiation.
Johnson TI, Costa ASH, Ferguson AN, Frezza C
Cell death & disease 2018 Sep 6;9(9):913
Cell death & disease 2018 Sep 6;9(9):913
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Western blot analysis of extracts (35ug) from 9 different cell lines by usin g anti-FH monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).
- Validation comment
- WB
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY FH (RC200614, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-FH.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Anti-FH mouse monoclonal antibody (TA500681) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY FH(RC200614).
- Validation comment
- IF
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- Immunofluorescent staining of HeLa cells using anti-FH mouse monoclonal antibody (TA500681).
- Validation comment
- IF
