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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- PA5-22091 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Fumarase Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, mosue liver, rat liver. Predicted reactivity: Rat (96%), Zebrafish (86%), Pig (97%), Chicken (89%), Sheep (97%), Chimpanzee (99%), Bovine (96%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Molecular and clinicopathologic characterization of intravenous leiomyomatosis.
Ordulu Z, Chai H, Peng G, McDonald AG, De Nictolis M, Garcia-Fernandez E, Hardisson D, Prat J, Li P, Hui P, Oliva E, Buza N
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2020 Sep;33(9):1844-1860
Modern pathology : an official journal of the United States and Canadian Academy of Pathology, Inc 2020 Sep;33(9):1844-1860
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using Fumarase Polyclonal Antibody (Product # PA5-22091). Sample (30 µg of whole cell lysate). Lane A: 293T. Lane B: A431. 10% SDS PAGE. Fumarase Polyclonal Antibody (Product # PA5-22091) diluted at 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fumarase Polyclonal Antibody detects FH protein by western blot analysis. A. 50 µg mouse liver lysate/extract. 10% SDS-PAGE. Fumarase Polyclonal Antibody (Product # PA5-22091) dilution: 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fumarase Polyclonal Antibody detects Fumarate hydratase protein by western blot analysis. A.50 µg rat liver lysate/extract.10% SDS-PAGE. Fumarase Polyclonal Antibody (Product # PA5-22091) dilution: 1:5,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of Fumarase was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: Fumarase Polyclonal Antibody (Product # PA5-22091) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded OVCAR3 xenograft, using fumarate hydratase (Product # PA5-22091) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
