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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
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- Product number
- PPS004 - Provider product page
- Provider
- R&D Systems
- Product name
- Phospho-Dynamin (S774) Antibody
- Antibody type
- Polyclonal
- Description
- Antigen Affinity-purified. Human, mouse, rat, bovine, canine, chicken, and primate ~95 kDa Dynamin protein phosphorylated at S774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g., Amphiphysin and Synapsin).
- Reactivity
- Human, Mouse, Rat, Bovine, Canine, Chicken/Avian, Simian
- Host
- Sheep
- Conjugate
- Unconjugated
- Isotype
- IgG
- Vial size
- 100 uL
- Storage
- For long-term storage, = -20° C is recommended. Product is stable at = -20° C for at least 1 year.
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Supportive validation
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- R&D Systems (provider)
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- Experimental details
- Detection of Phospho-Dynamin (S774) by Western Blot. Western blot of rat brain (hippocampus) tissue lysate stimulated with forskolin showing specific immunolabeling of the ~95 kDa dynamin phosphorylated at S774 (Control). The phosphospecificity of this labeling is dem-onstrated by treatment with 1200 U of lambda Phosphatase ( lambda-PPase) for 30 minutes before being exposed to the Anti-Phospho-Dynamin (S774). The immunolabeling is completely eliminated by treatment with lambda-PPase.
