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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
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- Product number
- NB300-209 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB300-209, RRID:AB_2093238
- Product name
- Sheep Polyclonal Dynamin Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specific for the ~95k dynamin protein phosphorylated at Ser774. Labels the purified protein phosphorylated in vitro by cdk5 but not by PKC. Does not cross react with other purified substrates of cdk5 (e.g. amphiphysin and synapsin). Immunolabeling is blocked by lambda-phosphatase treatment.
- Reactivity
- Rat
- Host
- Sheep
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Cdk5 is essential for synaptic vesicle endocytosis.
Tan TC, Valova VA, Malladi CS, Graham ME, Berven LA, Jupp OJ, Hansra G, McClure SJ, Sarcevic B, Boadle RA, Larsen MR, Cousin MA, Robinson PJ
Nature cell biology 2003 Aug;5(8):701-10
Nature cell biology 2003 Aug;5(8):701-10
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Supportive validation
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- Novus Biologicals (provider)
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- Experimental details
- Western Blot: Dynamin [p Ser774] Antibody [NB300-209] - Rat hippocampal lysate stimulated with forskolin showing specific immunolabeling of the ~95k dynamin phosphorylated at Ser774 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the Anti-Ser774 dynamin. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
