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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunoprecipitation [1]
- Chromatin Immunoprecipitation [1]
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- Product number
- NBP2-20430 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal SMYD3 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMYD3 Antibody [NBP2-20430] - A. 30 ug 293T whole cell lysate/extract B. 30 ug whole cell lysate/extract of human SMYD3-transfected 293T cells (partial fragment) 10 % SDS-PAGE SMYD3 antibody dilution: 1:1000
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMYD3 Antibody [NBP2-20430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at a dilution of 1:1000.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: SMYD3 Antibody [NBP2-20430] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with SMYD3 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: SMYD3 Antibody [NBP2-20430] - Immunoprecipitation of SMYD3 protein from 293T whole cell extracts using 5 ug of SMYD3 antibody. Western blot analysis was performed using SMYD3 antibody. EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Chromatin Immunoprecipitation: SMYD3 Antibody [NBP2-20430] - Cross-linked ChIP was performed with HepG2 chromatin extract and 5 ug of either normal rabbit IgG or anti-SMYD3 antibody. The precipitated DNA was detected by PCR with primer set targeting to 5' flanking region of Nkx2.8..
