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- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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- Product number
- 659402 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#659402, RRID:AB_2563252
- Product name
- Purified anti-TICAM-2 (TRAM)
- Antibody type
- Monoclonal
- Antigen
- Full length human TICAM-2 recombinant protein expressed in E. coli.
- Description
- TICAM-2, also known as TRIF-related adaptor molecule (TRAM), is a TIR domain containing protein that was found to act as an adaptor molecule during LPS-induced innate immune response. Upon infection by Gram-negative bacteria, TLR4 specifically recognizes LPS and leads to the dimerization of the cytoplasmic TIR domains. The activated TLR4 subsequently recruits the adaptor protein complex containing both TICAM-1 and TICAM-2, resulting in the activation of IFN response factors and production of pro-inflammatory cytokines. In this signaling cascade, TICAM-2 is essential for bridging the interaction between TLR4 and TICAM-1. TICAM-2 is also involved in the regulation of the IL-18 signaling pathway, acting as a sorting adaptor that recruits MYD88 from the cytosol to the membrane region upon activation of IL-1/IL-18 receptors.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antibody clone number
- 9G1B30
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
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- Experimental details
- Total cell lysate from THP-1 cells was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-TICAM2 (clone 9G1B30) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection.
- Conjugate
- Unconjugated
