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ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Product number
- NBP1-28748 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-28748, RRID:AB_1913962
- Product name
- Rabbit Polyclonal NUP188 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Nucleoporin Nup155 is part of the p53 network in liver cancer.
Holzer K, Ori A, Cooke A, Dauch D, Drucker E, Riemenschneider P, Andres-Pons A, DiGuilio AL, Mackmull MT, Baßler J, Roessler S, Breuhahn K, Zender L, Glavy JS, Dombrowski F, Hurt E, Schirmacher P, Beck M, Singer S
Nature communications 2019 May 14;10(1):2147
Nature communications 2019 May 14;10(1):2147
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Supportive validation
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- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: NUP188 Antibody [NBP1-28748] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NUP188 antibody NBP1-28748 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 10 ug/mg lysate. NUP188 was also immunoprecipitated by rabbit anti-NUP188 antibody NBP1-28717, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
