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LearnPA5-82767
antibody from Invitrogen Antibodies
Targeting: SEPTIN4
ARTS, C17orf47, CE5B3, FLJ40121, H5, hCDCREL-2, hucep-7, MART, PNUTL2, SEPT4
Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
- Other assay [2]
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- Product number
- PA5-82767 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- ARTS Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- Immunogen sequence: YDDDLEFRPP SRPQSSDNQQ YFCAPAPLSP SARPRSPWGK LDPYDSSEDD KEYVGFATLP NQVHRKS
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Peiminine Reduces ARTS-Mediated Degradation of XIAP by Modulating the PINK1/Parkin Pathway to Ameliorate 6-Hydroxydopamine Toxicity and α-Synuclein Accumulation in Parkinson's Disease Models In Vivo and In Vitro.
Hsu YL, Hung HS, Tsai CW, Liu SP, Chiang YT, Kuo YH, Shyu WC, Lin SZ, Fu RH
International journal of molecular sciences 2021 Sep 23;22(19)
International journal of molecular sciences 2021 Sep 23;22(19)
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARTS by a ARTS polyclonal antibody (Product # PA5-82767). Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of ARTS by a ARTS polyclonal antibody (Product # PA5-82767). Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11 Lane 2: Human cell line RT-4 Lane 3: Human cell line U-251MG sp Lane 4: Human plasma (IgG/HSA depleted) Lane 5: Human liver tissue Lane 6: Human tonsil tissue.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ARTS in human cerebral cortex and pancreas tissues using a ARTS polyclonal antibody (Product # PA5-82767). Corresponding RNA-seq data are presented for the same tissues.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ARTS in human cerebral cortex using a ARTS polyclonal antibody (Product # PA5-82767). The analysis shows high expression.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of ARTS in human pancreas using a ARTS polyclonal antibody (Product # PA5-82767). The analysis shows low expression as expected.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 10 Western blot analysis was used to quantify the levels of PINK1, parkin, ARTS, XIAP, and apoptotic core proteins. SH-SY5Y cells were transfected with control siRNA or parkin siRNA for 24 h, and then the transfected cells were treated with 1 muM PMN or no treatment, respectively. After 24 h, cells were exposed to 100 muM 6-OHDA for 12 h. ( a ) Shows representative results from one of three independent experiments. ( b ) ImageJ software was utilized to quantify the signal intensity of the image in ( a ). The loaded internal control is the level of beta-tubulin. The relative ratio is represented as the ratio of each group to the control group. # Indicates a significant difference between the 6-OHDA-exposed group and the control group ( # p < 0.001). * Indicates a significant difference between the PMN-pretreated 6-OHDA-exposed group and PMN-untreated 6-OHDA-exposed group (* p < 0.001).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 12 Western blot analysis was used to quantify the protein levels of PINK1, parkin, ARTS, XIAP, and autophagy core proteins. alpha-Synuclein-overexpressing SH-SY5Y cells were transfected with control siRNA or parkin siRNA for 24 h. Next, the transfected cells were treated with 1 muM PMN or untreated for 24 h and then analyzed. ( a ) Shows representative results from one of three independent experiments. ( b ) ImageJ software was utilized to quantify the signal intensity of the image in ( a ). The loaded internal control was the level of beta-tubulin. The relative ratio is represented as the ratio of each group to the control group. # Indicates a significant difference between the alpha-synuclein-overexpressing group and the control group ( # p < 0.001). * Indicates a significant difference between the PMN-treated group and the PMN-untreated group (* p < 0.001).
