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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Western blot [3]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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- Product number
- GTX100874 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100874, RRID:AB_1949749
- Product name
- BMP4 antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
Submitted references Decrease of FSTL1-BMP4-Smad signaling predicts poor prognosis in lung adenocarcinoma but not in squamous cell carcinoma.
Chiou J, Su CY, Jan YH, Yang CJ, Huang MS, Yu YL, Hsiao M
Scientific reports 2017 Aug 29;7(1):9830
Scientific reports 2017 Aug 29;7(1):9830
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- BMP4 antibody detects BMP4 protein by western blot analysis.A. 30 ?g A549 whole cell lysate/extract10% SDS-PAGEBMP4 antibody (GTX100874) dilution: 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A: NIH-3T3 B: JC 10% SDS PAGE GTX100874 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- HepG2 whole cell extract and conditioned medium (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with BMP4 antibody (GTX100874) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of methanol-fixed HeLa, using BMP4(GTX100874) antibody at 1:100 dilution.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4(GTX100874) antibody at 1:100 dilution.
