Antibody data
- Antibody Data
- Antigen structure
- References [10]
- Comments [0]
- Validations
- Blocking/Neutralizing [1]
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- Product number
- MAB757-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human BMP-4 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from ascites. Detects human BMP-4 in direct ELISAs and Western blots. This antibody shows approximately 5% cross-reactivity with recombinant human BMP-2.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
P12644
- Isotype
- IgG
- Antibody clone number
- 66119
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Submitted references Furin, a transcriptional target of NKX2-5, has an essential role in heart development and function.
A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma.
Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer.
Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells.
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
Dupays L, Towers N, Wood S, David A, Stuckey DJ, Mohun T
PloS one 2019;14(3):e0212992
PloS one 2019;14(3):e0212992
A subtype of cancer-associated fibroblasts with lower expression of alpha-smooth muscle actin suppresses stemness through BMP4 in oral carcinoma.
Patel AK, Vipparthi K, Thatikonda V, Arun I, Bhattacharjee S, Sharan R, Arun P, Singh S
Oncogenesis 2018 Oct 5;7(10):78
Oncogenesis 2018 Oct 5;7(10):78
Contribution of three-dimensional architecture and tumor-associated fibroblasts to hepcidin regulation in breast cancer.
Blanchette-Farra N, Kita D, Konstorum A, Tesfay L, Lemler D, Hegde P, Claffey KP, Torti FM, Torti SV
Oncogene 2018 Jul;37(29):4013-4032
Oncogene 2018 Jul;37(29):4013-4032
Thrombospondin-1 (TSP-1), a new bone morphogenetic protein-2 and -4 (BMP-2/4) antagonist identified in pituitary cells.
Sallon C, Callebaut I, Boulay I, Fontaine J, Logeart-Avramoglou D, Henriquet C, Pugnière M, Cayla X, Monget P, Harichaux G, Labas V, Canepa S, Taragnat C
The Journal of biological chemistry 2017 Sep 15;292(37):15352-15368
The Journal of biological chemistry 2017 Sep 15;292(37):15352-15368
A rapid and sensitive bioassay for the simultaneous measurement of multiple bone morphogenetic proteins. Identification and quantification of BMP4, BMP6 and BMP9 in bovine and human serum.
Herrera B, Inman GJ
BMC cell biology 2009 Mar 19;10:20
BMC cell biology 2009 Mar 19;10:20
Bone morphogenetic proteins promote gliosis in demyelinating spinal cord lesions.
Fuller ML, DeChant AK, Rothstein B, Caprariello A, Wang R, Hall AK, Miller RH
Annals of neurology 2007 Sep;62(3):288-300
Annals of neurology 2007 Sep;62(3):288-300
Activation of the BMP canonical signaling pathway in human optic nerve head tissue and isolated optic nerve head astrocytes and lamina cribrosa cells.
Zode GS, Clark AF, Wordinger RJ
Investigative ophthalmology & visual science 2007 Nov;48(11):5058-67
Investigative ophthalmology & visual science 2007 Nov;48(11):5058-67
Altered bone morphogenetic protein signalling in the Helicobacter pylori-infected stomach.
Bleuming SA, Kodach LL, Garcia Leon MJ, Richel DJ, Peppelenbosch MP, Reitsma PH, Hardwick JC, van den Brink GR
The Journal of pathology 2006 Jun;209(2):190-7
The Journal of pathology 2006 Jun;209(2):190-7
BMPER, a novel endothelial cell precursor-derived protein, antagonizes bone morphogenetic protein signaling and endothelial cell differentiation.
Moser M, Binder O, Wu Y, Aitsebaomo J, Ren R, Bode C, Bautch VL, Conlon FL, Patterson C
Molecular and cellular biology 2003 Aug;23(16):5664-79
Molecular and cellular biology 2003 Aug;23(16):5664-79
Sonic hedgehog regulates gastric gland morphogenesis in man and mouse.
van den Brink GR, Hardwick JC, Tytgat GN, Brink MA, Ten Kate FJ, Van Deventer SJ, Peppelenbosch MP
Gastroenterology 2001 Aug;121(2):317-28
Gastroenterology 2001 Aug;121(2):317-28
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Alkaline Phosphatase Production Induced by BMP-4 and Neutralization by Human BMP-4 Antibody. Recombinant Human BMP-4 (Catalog # 314-BP) induces alkaline phosphatase production in the the C2C12 mouse myoblast cell line in a dose-dependent manner (orange line). Alkaline phosphatase production elicited by Recombinant Human BMP-4 (500 ng/mL) is neutralized (green line) by increasing concentrations of Human BMP-4 Monoclonal Antibody (Catalog # MAB757). The ND50 is typically 1-3 µg/mL in the presence of heparin (2 µg/mL).