PA5-27288

antibody from Invitrogen Antibodies

Targeting: BMP4 BMP2B

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-27288

Provider

Invitrogen Antibodies

Product name

BMP-4 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: HepG2 conditioned medium, NIH-3T3, JC. Predicted reactivity: Mouse (97%), Rat (96%), Dog (97%), Pig (98%), Rabbit (96%), Sheep (98%), Bovine (97%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Reactivity

Human

Host

Rabbit

Isotype

IgG

Vial size

100 µL

Concentration

0.36 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Consecutive Hypoxia Decreases Expression of NOTCH3, HEY1, CC10, and FOXJ1 via NKX2-1 Downregulation and Intermittent Hypoxia-Reoxygenation Increases Expression of BMP4, NOTCH1, MKI67, OCT4, and MUC5AC via HIF1A Upregulation in Human Bronchial Epithelial Cells.

Yang YY, Lin CJ, Wang CC, Chen CM, Kao WJ, Chen YH
Frontiers in cell and developmental biology 2020;8:572276

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Nano-Polyplexes Mediated Transfection of Runx2-shRNA Mitigates the Osteodifferentiation of Human Valvular Interstitial Cells.

Voicu G, Rebleanu D, Constantinescu CA, Fuior EV, Ciortan L, Droc I, Uritu CM, Pinteala M, Manduteanu I, Simionescu M, Calin M
Pharmaceutics 2020 Jun 2;12(6)

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMP4 using 30 µg A549 whole cell lysate. Samples were loaded onto a 10% SDS-PAGE gel and probed with a BMP4 polyclonal antibody (Product # PA5-27288) at a dilution of 1:1000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMP-4 was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMP-4 Polyclonal Antibody (Product # PA5-27288) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of BMP-4 was performed by separating 30 µg of HepG2 whole cell extract and conditioned medium by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a BMP-4 Polyclonal Antibody (Product # PA5-27288) at a dilution of 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using BMP-4 Polyclonal Antibody (Product # PA5-27288). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. Lane B: JC. 10% SDS PAGE. BMP-4 Polyclonal Antibody (Product # PA5-27288) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of BMP4 in methanol-fixed HeLa cells using a BMP4 polyclonal antibody (Product # PA5-27288) at a 1:100 dilution.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of paraffin-embedded MDA-MB468 xenograft, using BMP4 (Product # PA5-27288) antibody at 1:100 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

FIGURE 9 Colocalization and concordant regulation of the immunofluorescence signals of HIF1A, BMP4 and OCT4 proteins, and of the immunofluorescence signals of NKX2-1, CC10 and HEY1 proteins in both undifferentiated NHBE and DHBE cells. (A-H) Triple immunofluorescence staining for HIF1A (green), BMP4 (magenta), and OCT4 (red) in the submerged cultures of undifferentiated NHBE cells (A-D) and DHBE cells (E-H) revealed colocalization of HIF1A, BMP4 and OCT4 proteins in the same HBE cells. (I-K) Statistical charts showing concordant upregulation of the immunofluorescence intensities of HIF1A, BMP4 and OCT4 proteins by intermittent H/R in both the NHBE and DHBE cells. (L-T) Triple immunofluorescence staining for NKX2-1 (green), CC10 (magenta) and HEY1 (red) in the submerged cultures of undifferentiated NHBE cells (L-P) and DHBE cells (Q-T) revealed colocalization of NKX2-1, CC10 and HEY1 proteins in the same HBE cells. (U-W) Statistical charts showing concordant downregulation of the immunofluorescence intensities of NKX2-1, CC10, and HEY1 proteins by consecutive hypoxia in both the NHBE and DHBE cells. The scale bars in (A,E,L,Q) all represent 50 mum and respectively apply to panels (A-H,L-T) .