13431-1-AP
antibody from Invitrogen Antibodies
Targeting: CYP51A1
CP51, CYP51, CYPL1, LDM, P450-14DM, P450L1
Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [7]
- Immunocytochemistry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- 13431-1-AP - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CYP51A1 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Immunogen sequence: GKGVAYDVP NPVFLEQKKM LKSGLNIAHF KQHVSIIEKE TKEYFESWGE SGEKNVFEAL SELIILTASH CLHGKEIRSQ LNEKVAQLYA DLDGGFSHAA WLLPGWLPLP SFRRRDRAHR EIKDIFYKAI QKRRQSQEKI DDILQTLLDA TYKDGRPLTD DEVAGMLIGL LLAGQHTSST TSAWMGFFLA RDKTLQKKCY LEQKTVCGEN LPPLTYDQLK DLNLLDRCIK ETLRLRPPVM IMMRMARTPQ TVAGYTIPPG HQVCVSPTVN QRLKDSWVER LDFNPDRYLQ DNPASGEKFA YVPFGAGRHR CIGENFAYVQ IKTIWSTMLR LYEFDLIDGY FPTVNYTTMI HTPENPVIRY KRRSK (146-509 aa encoded by BC032322)
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 150 µL
- Concentration
- 0.13 mg/mL
- Storage
- -20°C
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse testis tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- PC-3 cells were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse ovary tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- HL-60 cells were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Mouse lung tissue were subjected to SDS PAGE followed by western blot with 13431-1-AP (CYP51A1 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of ( -20°C Ethanol ) fixed PC-3 cells using 13431-1-AP (CYP51A1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IP result of anti-CYP51A1 (IP:13431-1-AP, 4ug; Detection:13431-1-AP 1:1000) with mouse testis tissue lysate 2800ug.