- GTX101507 - GeneTex SOX2 antibody

Submitted references Lead exposure reduces survival, neuronal determination, and differentiation of P19 stem cells.

Mansel C, Fross S, Rose J, Dema E, Mann A, Hart H, Klawinski P, Vohra BPS
Neurotoxicology and teratology 2019 Mar - Apr;72:58-70

ACTL6A interacts with p53 in acute promyelocytic leukemia cell lines to affect differentiation via the Sox2/Notch1 signaling pathway.

Zhong PQ, Zhong L, Yao JJ, Liu DD, Yuan Z, Liu JM, Chen M, Yao SF, Zhao Y, Liu L, Li LW, Liu BZ
Cellular signalling 2019 Jan;53:390-399

Cross Talk between One-Carbon Metabolism, Eph Signaling, and Histone Methylation Promotes Neural Stem Cell Differentiation.

Fawal MA, Jungas T, Kischel A, Audouard C, Iacovoni JS, Davy A
Cell reports 2018 Jun 5;23(10):2864-2873.e7

Embryos and embryonic stem cells from the white rhinoceros.

Hildebrandt TB, Hermes R, Colleoni S, Diecke S, Holtze S, Renfree MB, Stejskal J, Hayashi K, Drukker M, Loi P, Göritz F, Lazzari G, Galli C
Nature communications 2018 Jul 4;9(1):2589

The Sox2 promoter-driven CD63-GFP transgenic rat model allows tracking of neural stem cell-derived extracellular vesicles.

Yoshimura A, Adachi N, Matsuno H, Kawamata M, Yoshioka Y, Kikuchi H, Odaka H, Numakawa T, Kunugi H, Ochiya T, Tamai Y
Disease models & mechanisms 2018 Jan 30;11(1)

MDM2 Degrades Deacetylated Nucleolin Through Ubiquitination to Promote Glioma Stem-Like Cell Enrichment for Chemotherapeutic Resistance.

Ko CY, Lin CH, Chuang JY, Chang WC, Hsu TI
Molecular neurobiology 2018 Apr;55(4):3211-3223

Nodal signaling activates the Smad2/3 pathway to regulate stem cell-like properties in breast cancer cells.

Gong W, Sun B, Sun H, Zhao X, Zhang D, Liu T, Zhao N, Gu Q, Dong X, Liu F
American journal of cancer research 2017;7(3):503-517

Human Deciduous Teeth Stem Cells (SHED) Display Neural Crest Signature Characters.

Gazarian KG, Ramírez-García LR
PloS one 2017;12(1):e0170321

Enforcement of developmental lineage specificity by transcription factor Oct1.

Shen Z, Kang J, Shakya A, Tabaka M, Jarboe EA, Regev A, Tantin D
eLife 2017 May 24;6

Antroquinonol, a Ubiquinone Derivative from the Mushroom Antrodia camphorata, Inhibits Colon Cancer Stem Cell-like Properties: Insights into the Molecular Mechanism and Inhibitory Targets.

Lin HC, Lin MH, Liao JH, Wu TH, Lee TH, Mi FL, Wu CH, Chen KC, Cheng CH, Lin CW
Journal of agricultural and food chemistry 2017 Jan 11;65(1):51-59

The STAT3-miRNA-92-Wnt Signaling Pathway Regulates Spheroid Formation and Malignant Progression in Ovarian Cancer.

Chen MW, Yang ST, Chien MH, Hua KT, Wu CJ, Hsiao SM, Lin H, Hsiao M, Su JL, Wei LH
Cancer research 2017 Apr 15;77(8):1955-1967

Germline Competent Pluripotent Mouse Stem Cells Generated by Plasmid Vectors.

Chen CH, Su YH, Lee KH, Chuang CK
Animal biotechnology 2016;27(3):157-65

Activation of Sphingosine 1-Phosphate Receptor 1 Enhances Hippocampus Neurogenesis in a Rat Model of Traumatic Brain Injury: An Involvement of MEK/Erk Signaling Pathway.

Ye Y, Zhao Z, Xu H, Zhang X, Su X, Yang Y, Yu X, He X
Neural plasticity 2016;2016:8072156

Derivation of Patient Specific Pluripotent Stem Cells Using Clinically Discarded Cumulus Cells.

Chang WF, Hwu YM, Xu J, Lin CJ, Wang SW, Cheng AS, Lu J, Lu CH, Sung LY
PloS one 2016;11(11):e0165715

Induced Pluripotent Stem Cell Differentiation and Three-Dimensional Tissue Formation Attenuate Clonal Epigenetic Differences in Trichohyalin.

Petrova A, Capalbo A, Jacquet L, Hazelwood-Smith S, Dafou D, Hobbs C, Arno M, Farcomeni A, Devito L, Badraiq H, Simpson M, McGrath JA, Di WL, Cheng JB, Mauro TM, Ilic D
Stem cells and development 2016 Sep 15;25(18):1366-75

PAK1 confers chemoresistance and poor outcome in non-small cell lung cancer via β-catenin-mediated stemness.

Chen MJ, Wu DW, Wang YC, Chen CY, Lee H
Scientific reports 2016 Oct 7;6:34933

Suberoylanilide hydroxamic acid represses glioma stem-like cells.

Hsu CC, Chang WC, Hsu TI, Liu JJ, Yeh SH, Wang JY, Liou JP, Ko CY, Chang KY, Chuang JY
Journal of biomedical science 2016 Nov 18;23(1):81

Cops2 promotes pluripotency maintenance by Stabilizing Nanog Protein and Repressing Transcription.

Zhang W, Ni P, Mou C, Zhang Y, Guo H, Zhao T, Loh YH, Chen L
Scientific reports 2016 May 26;6:26804

Inhibitor of Differentiation-1 and Hypoxia-Inducible Factor-1 Mediate Sonic Hedgehog Induction by Amyloid Beta-Peptide in Rat Cortical Neurons.

Hung YH, Chang SH, Huang CT, Yin JH, Hwang CS, Yang LY, Yang DI
Molecular neurobiology 2016 Mar;53(2):793-809

Roles of Klf5 Acetylation in the Self-Renewal and the Differentiation of Mouse Embryonic Stem Cells.

Zhao T, Liu C, Chen L
PloS one 2015;10(9):e0138168

Erk signaling is indispensable for genomic stability and self-renewal of mouse embryonic stem cells.

Chen H, Guo R, Zhang Q, Guo H, Yang M, Wu Z, Gao S, Liu L, Chen L
Proceedings of the National Academy of Sciences of the United States of America 2015 Nov 3;112(44):E5936-43

Therapeutic Potential of Human Adipose-Derived Stem/Stromal Cell Microspheroids Prepared by Three-Dimensional Culture in Non-Cross-Linked Hyaluronic Acid Gel.

Mineda K, Feng J, Ishimine H, Takada H, Doi K, Kuno S, Kinoshita K, Kanayama K, Kato H, Mashiko T, Hashimoto I, Nakanishi H, Kurisaki A, Yoshimura K
Stem cells translational medicine 2015 Dec;4(12):1511-22

Selective anticancer effects of a synthetic flavagline on human Oct4-expressing cancer stem-like cells via a p38 MAPK-dependent caspase-3-dependent pathway.

Emhemmed F, Ali Azouaou S, Thuaud F, Schini-Kerth V, Désaubry L, Muller CD, Fuhrmann G
Biochemical pharmacology 2014 May 15;89(2):185-96

Yin Yang 1 is a target of microRNA-34 family and contributes to gastric carcinogenesis.

Wang AM, Huang TT, Hsu KW, Huang KH, Fang WL, Yang MH, Lo SS, Chi CW, Lin JJ, Yeh TS
Oncotarget 2014 Jul 15;5(13):5002-16

Quantitative proteomic analysis of sphere-forming stem-like oral cancer cells.

Misuno K, Liu X, Feng S, Hu S
Stem cell research & therapy 2013;4(6):156

No comments: Submit comment

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ug of whole cell lysate) A: human ESC 10% SDS PAGE GTX101507 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: NT2D1 10% SDS PAGE GTX101507 diluted at 1:3000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (20 ug of whole cell lysate) A: mouse ESC 12% SDS PAGE GTX101507 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Sample (30 ug of whole cell lysate) A: A549 10% SDS PAGE GTX101507 diluted at 1:1000
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by western blot analysis.A. 30 ?g mouse ESC whole cell lysate/extract10 % SDS-PAGESOX2 antibody (GTX101507) dilution: 1:2500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by western blot analysis.A. 30 ?g mouse ESC whole cell lysate/extract10 % SDS-PAGESOX2 antibody (GTX101507) dilution: 1:2500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by western blot analysis.A. 30 ?g human ESC whole cell lysate/extract10 % SDS-PAGESOX2 antibody (GTX101507) dilution: 1:2500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by western blot analysis.A. 30 ?g mouse ESC whole cell lysate/extract10 % SDS-PAGESOX2 antibody (GTX101507) dilution: 1:2500
Validation comment: WB

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membranes were blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000 and competitor's antibody (sc-17320) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.*The competitor is not affiliated with GeneTex and does not endorse this product.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Non-transfected (¡V) and transfected (+) NT2D1 whole cell extracts (30 ?g) were separated by 10% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:15000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:2500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody detects SOX2 protein by Western blot analysis. Whole cell extracts (30 £gg) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody (GTX101507) at a dilution of 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Whole cell extract (30 ?g) was separated by 12% SDS-PAGE, and the membrane was blotted with SOX2 antibody [N1C3] (GTX101507) diluted at 1:10000.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed mouse ESC, using SOX2(GTX101507) antibody at 1:80 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunofluorescence analysis of paraformaldehyde-fixed Human ESC, using SOX2(GTX101507) antibody at 1:160 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: A549 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: Phalloidin, a cytoskeleton marker, diluted at 1:200.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2/D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000.Red: Phalloidin, a cytoskeleton marker, diluted at 1:50.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunofluorescent analysis.Sample: NT2D1 cells were fixed in 4% paraformaldehyde at RT for 15 min.Green: SOX2 stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.Red: phalloidin, a cytoskeleton? marker, diluted at 1:100.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] immunoprecipitates SOX2 protein in IP experiments.IP samples: NT2D1 nuclear extractA. 50 ?g NT2D1 nuclear extractB. Control with 4 ?g of preimmune Rabbit IgGC. Immunoprecipitation of SOX2 protein by 4 ?g SOX2 antibody [N1C3] (GTX101507)10 % SDS-PAGEThe immunoprecipitated SOX2 protein was detected by SOX2 antibody [N1C3] (GTX101507) diluted at 1:1000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
Validation comment: IP

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 £gg of SOX2 antibody [N1C3] (GTX101507) or SOX2 antibody [GT1876] (GTX627404).Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunoprecipitation of SOX2 protein from NT2D1 whole cell extracts using 5 £gg of SOX2 antibody [N1C3] (GTX101507).Western blot analysis was performed using SOX2 antibody [N1C3] (GTX101507).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using SOX2(GTX101507) antibody at 1:100 dilution.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus on rat brain stem by immunohistochemical analysis. Sample: Paraffin-embedded rat brain stem. SOX2 antibody [N1C3] (GTX101507) dilution: 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus on mouse fore brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fore brain. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned E13.5 rat brain. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.Red: beta Tubulin 3/ TUJ1, a mature neuron marker, stained by beta Tubulin 3/ TUJ1 antibody [GT11710] (GTX631836) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus by immunohistochemical analysis.Sample: Frozen sectioned adult mouse retina. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.Red: Protein kinase C alpha staining.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse esophagus by immunohistochemical analysis. Sample: Paraffin-embedded mouse esophagus. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus in mouse fetal brain by immunohistochemical analysis. Sample: Paraffin-embedded mouse fetal brain. Green: SOX2 antibody [N1C3] (GTX101507) diluted at 1:200. The signal was developed using goat anti-rabbit IgG antibody (Dylight488) (GTX213110-04).Blue: Nuclear staining with Hoechst 33342.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at cytoplasm and nucleus in human esophagus by immunohistochemical analysis. Sample: Paraffin-embedded human esophagus. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human cervical carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human cervical carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein at nucleus in human esophageal carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human esophageal carcinoma. SOX2 antibody [N1C3] (GTX101507) diluted at 1:500.

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: SOX2 antibody [N1C3] detects SOX2 protein expression by immunohistochemical analysis.Sample: Frozen-sectioned adult mouse hippocampus. Green: SOX2 protein stained by SOX2 antibody [N1C3] (GTX101507) diluted at 1:250.Blue: Fluoroshield with DAPI (GTX30920).

Supportive validation

Submitted by: GeneTex (provider)
Main image
Experimental details: Flow cytometry on human embryonic stem cells, staining with SOX2 (GTX101507)antibody at 1:100 dilution(purple) or rabbit IgG (black).

GTX101507

Antibody data