Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Blocking/Neutralizing [1]
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Validation data
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- Product number
- MAB94182-100 - Provider product page
- Provider
- R&D Systems
- Product name
- Human VSIG8 Antibody
- Antibody type
- Monoclonal
- Description
- Protein A or G purified from hybridoma culture supernatant. Detects human VSIG8 in direct ELISAs.
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
Q5VU13
- Isotype
- IgG
- Antibody clone number
- 961829
- Vial size
- 100 ug
- Storage
- Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution. 6 months, -20 to -70 °C under sterile conditions after reconstitution.
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Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- Detection of VSIG8 in HEK293 Human Cell Line Transfected with Human VSIG8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) human VSIG8 or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94182) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB0041). View our protocol for Staining Membrane-associated Proteins.
Supportive validation
- Submitted by
- R&D Systems (provider)
- Main image
- Experimental details
- VISTA/B7-H5 Binding to VSIG8-transfected HEK293 Human Cell Line is Blocked by Human VSIG8 Antibody. In a functional flow cytometry test, recombinant human VISTA/B7-H5 (20 ng/mL, Catalog # 7126-B7) binds to HEK293 human embryonic kidney cell line transfected with human VSIG8 and eGFP. (A) Binding is completely blocked by 2.5 μg/mL of Mouse Anti-Human VSIG8 Monoclonal Antibody (Catalog # MAB94182) but not by (B) Mouse IgG2B Isotype Control (Catalog # MAB0041). Binding of recombinant human B7-H5/VISTA on cells is detected with anti-Human IgG Fc APC-conjugated Monoclonal Antibody (Catalog # FAB110A).