PA5-66513

antibody from Invitrogen Antibodies

Targeting: MSANTD3 C9orf30, MGC17337

Immunocytochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-66513

Provider

Invitrogen Antibodies

Product name

MSANTD3 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant full-length protein

Description

Immunogen sequence: MNEVHVAKIQQ IERECEMAEE EHRIKMEVLN KKKMYWERKL QTFTKEWPVS SFNRPFPNSP

Concentration

0.1 mg/mL

References

Submitted references

Oncogenic Orphan Nuclear Receptor NR4A3 Interacts and Cooperates with MYB in Acinic Cell Carcinoma.

Lee DY, Brayer KJ, Mitani Y, Burns EA, Rao PH, Bell D, Williams MD, Ferrarotto R, Pytynia KB, El-Naggar AK, Ness SA
Cancers 2020 Aug 27;12(9)

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of MSANTD3 in human cell line RH-30 shows localization to nucleoplasm. Samples were probed using a MSANTD3 Polyclonal Antibody (Product # PA5-66513).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 4 NR4A3 and MYB cooperate and interact. ( A ) Western blot analysis. Sample identifiers are shown at top, antibodies used to probe the blot are indicated at right. An actin loading control is shown at the bottom. The fusions that were detected ('+') or not detected ('-') by either RNA-seq or FISH are indicated along the bottom (blanks were not tested). High expression of NR4A3 protein was detected in all samples with t(4;9), but not in the normal samples (right) or the sample with t(2;4), which expressed the NR4A2 gene instead. Two distinct NR4A3 proteins were detected; the main form (approximately 68kD, lower band) and an isoform (approximately 80kD, upper band). MSANTD3 protein was detected in the two samples that also had fusions activating that gene (390A2 and 670H1); ( B ) NR4A3 and Myb cooperate. HeLa cells were transfected with the NuRE promoter-luciferase vector plus plasmids expressing NR4A3 alone or with different Myb constructs, as indicated. The Myb proteins are diagrammed at the bottom. Additional controls are in Figure S5 ; ( C ) NR4A3 pull-down assays. The NR4A3 protein is diagrammed at top, including the DNA binding (red) and ligand binding (blue) domains. GST fusion proteins used for pull-down assays are diagrammed. Left panel: Cos-7 cells were co-transfected with plasmids expressing HA-tagged NR4A3 or Myb, protein complexes were immunoprecipitated using control IgG or anti-HA tag antibodies, and the complexes were detected by western blot using anti-M