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- Immunohistochemistry [2]
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- Product number
- NBP1-76695 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76695, RRID:AB_11036308
- Product name
- Rabbit Polyclonal TWEAK/TNFSF12 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Tumor necrosis factor-like weak inducer of apoptosis expression in healthy oral mucosa, oral dysplasia and oral squamous cell carcinoma.
TWEAK protects cardiomyocyte against apoptosis in a PI3K/AKT pathway dependent manner.
The inflammatory cytokine TWEAK decreases PGC-1α expression and mitochondrial function in acute kidney injury.
Acharya S, Prabhu P, Patil V, Acharya AB, Desai A, Nikhil K
Journal of oral and maxillofacial pathology : JOMFP 2019 Sep-Dec;23(3):369-377
Journal of oral and maxillofacial pathology : JOMFP 2019 Sep-Dec;23(3):369-377
TWEAK protects cardiomyocyte against apoptosis in a PI3K/AKT pathway dependent manner.
Yang B, Yan P, Gong H, Zuo L, Shi Y, Guo J, Guo R, Xie J, Li B
American journal of translational research 2016;8(9):3848-3860
American journal of translational research 2016;8(9):3848-3860
The inflammatory cytokine TWEAK decreases PGC-1α expression and mitochondrial function in acute kidney injury.
Ruiz-Andres O, Suarez-Alvarez B, Sánchez-Ramos C, Monsalve M, Sanchez-Niño MD, Ruiz-Ortega M, Egido J, Ortiz A, Sanz AB
Kidney international 2016 Feb;89(2):399-410
Kidney international 2016 Feb;89(2):399-410
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Analysis of TWEAK in HeLa cell lysate with TWEAK antibody at (A) 1 and (B) 2 ug/mL.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Liver Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation in Mouse Pancreas Tissue Lysates. Loading: 15 ug of lysates per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 1 ug/mL and Lane 2: 2 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: TWEAK/TNFSF12 Antibody [NBP1-76695] - Western Blot Validation with Recombinant Protein. Loading: 30 ng of human TWEAK recombinant protein per lane. Antibodies: TWEAK/TNFSF12 NBP1-76695 (Lane 1: 0.5 ug/mL, Lane 2: 1 ug/mL, Lane 3: 2 ug/mL and Lane 4: 4 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat antirabbit IgG HRP conjugate at 1:10000 dilution. Observed at around 18 kDa.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: TWEAK/TNFSF12 Antibody [NBP1-76695] - Human brain tissue with TWEAK antibody at 10 ug/ml.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: TWEAK/TNFSF12 Antibody [NBP1-76695] - Staining of TWEAK in mouse brain tissue with TWEAK antibody at 5 ug/mL.
