Explore
Validate
LearnMA5-23848
antibody from Invitrogen Antibodies
Targeting: IL10RB
CDW210B, CRF2-4, CRFB4, D21S58, D21S66, IL-10R2
Flow cytometryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Flow cytometry [1]
- Other assay [1]
Submit
Validation data
Reference
Comment
Report error
- Product number
- MA5-23848 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IL10RB Monoclonal Antibody (90220)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-10 R alpha, rhIFN-gamma RI, and rhIFN-gamma R2 is observed.
- Antibody clone number
- 90220
- Concentration
- 0.5 mg/mL
No comments: Submit comment
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of IL10RB in Human PBMC monocytes. Samples were incubated in IL10RB monoclonal antibody (Product # MA5-23848) or isotype control antibody followed by PE-conjugated Anti-Mouse IgG Secondary Antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of IL10RB in LPS-activated human peripheral blood mononuclear cells (PBMC). Samples were incubated in IL10RB monoclonal antibody (Product # MA5-23848). Recombinant Human IL‚10 inhibits IL‚1β secretion in LPS-activated human peripheral blood mononuclear cells (PBMC) in a dose-dependent manner (orange line), as measured by the Human IL‚1β/IL‚1F2 Quantikine ELISA Kit. IL‚1β secretion inhibited by Recombinant Human IL‚10 (0.25 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL‚10 Rβ Monoclonal Antibody. The ND50 is typically 0.5-2 µg/mL in the presence of LPS (0.25 ng/mL).
