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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- MA5-14482 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- CD3e Monoclonal Antibody (EP449E)
- Antibody type
- Monoclonal
- Antigen
- Synthetic peptide
- Description
- MA5-14482 targets CD3E in IHC (P) and Western blot applications and shows reactivity with Human and Mouse samples. The MA5-14482 immunogen is a synthetic peptide corresponding to residues in the cytoplasmic domain of human CD3epsilon. The antibody does not cross react with other subunits of CD3.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- EP449E
- Vial size
- 1 mL
- Concentration
- Conc. Not Determined
- Storage
- 4° C
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of CD3E was performed by loading 25 µg of Jurkat (Lane 1), CEM (Lane 2) and mouse spleen (Lane 3) cell lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with a CD3E monoclonal antibody (Product # MA5-14482) at a dilution of 1:20 overnight at 4°C, washed in TBST, and probed with an HRP-conjugated secondary antibody for 1 hr at room temperature in the dark. Chemiluminescent detection was performed using Pierce ECL Plus Western Blotting Substrate (Product # 32132). Results show a band at approx. 20 kDa.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Formalin-fixed, paraffin-embedded human tonsil stained with CD3 Epsilon using peroxidase-conjugate and DAB chromogen. Note membrane staining.
