Antibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [5]
Submit
Validation data
Reference
Comment
Report error
- Product number
- NBP1-87886 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-87886, RRID:AB_11021814
- Product name
- Rabbit Polyclonal JM4 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified. Specificity of human, rat JM4 antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Expression profile of PRAF2 in the human brain and enrichment in synaptic vesicles.
Koomoa DL, Go RC, Wester K, Bachmann AS
Neuroscience letters 2008 May 9;436(2):171-6
Neuroscience letters 2008 May 9;436(2):171-6
No comments: Submit comment
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JM4 Antibody [NBP1-87886] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells). Lane 2: NBT-II cell lysate (Rat Wistar bladder tumor cells). Lane 3: PC12 cell lysate (Pheochromocytoma of rat adrenal medulla).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: JM4 Antibody [NBP1-87886] - Lane 1: Marker [kDa] 206, 113, 82, 49, 32, 26, 18. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Analysis in human cerebral cortex and skeletal muscle tissues. Corresponding PRAF2 RNA-seq data are presented for the same tissues.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human testis shows moderate to strong cytoplasmic positivity in cells in seminiferous ducts.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human cerebral cortex shows moderate to strong cytoplasmic positivity in neurons.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human skeletal muscle shows no positivity in myocytes as expected.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: JM4 Antibody [NBP1-87886] - Staining of human cerebellum shows moderate to strong cytoplasmic positivity in Purkinje cells.