10518-2-AP

antibody from Invitrogen Antibodies

Targeting: PACSIN2 SDPII

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

10518-2-AP

Provider

Invitrogen Antibodies

Product name

PACSIN2 Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Other

Description

Immunogen sequence: PSLNPEQLK KLQDKIEKCK QDVLKTKEKY EKSLKELDQG TPQYMENMEQ VFEQCQQFEE KRLRFFREVL LEVQKHLDLS NVAGYKAIYH DLEQSIRAAD AVEDLRWFRA NHGPGMAMNW PQFEEWSADL NRTLSRREKK KATDGVTLTG INQTGDQSLP SKPSSTLNVP SNPAQSAQSQ SSYNPFEDED DTGSTVSEKD DTKAKNVSSY EKTQSYPTDW SDDESNNPFS STDANGDSNP FDDDATSGTE VRVRALYDYE GQEHDELSFK AGDELTKMED EDEQGWCKGR LDNGQVGLYP ANYVEAIQ (180-486 aa encoded by BC008037)

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

150 µL

Concentration

0.2 mg/mL

Storage

-20°C

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse lung tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Mouse heart tissue were subjected to SDS PAGE followed by western blot with 10518-2-AP (PACSIN2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10518-2-AP ( PACSIN2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescent analysis of ( 10% Formaldehyde ) fixed NIH/3T3 cells using 10518-2-AP (PACSIN2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IGG (H+L).

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human skin tissue slide using 10518-2-AP (PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer using 10518-2-AP (PACSIN2 antibody) at dilution of 1:100 (under 25x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10518-2-AP ( PACSIN2 Antibody) at dilution of 1:400 (under 10x lens).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry of paraffin-embedded human breast cancer tissue slide using 10518-2-AP ( PACSIN2 Antibody) at dilution of 1:400 (under 40x lens).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

IP result of anti-PACSIN2 (IP:10518-2-AP, 4ug; Detection:10518-2-AP 1:500) with NIH/3T3 cells lysate 1200ug.