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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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- Product number
- PA5-121253 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- FRMD6 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive test controls include: SH-SY5Y, HeLa, HepG2, MCF7, Mouse kidney, OVCAR8, Rat lung, Rat spleen. The target is usually found in the following locations: Cell membrane, Cytoplasm, Cytoplasmic side, Peripheral membrane protein.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1.53 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FRMD6 in various cell lines using a FRMD6 Polyclonal antibody (Product # PA5-121253) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of FRMD6 in various cell lines using a FRMD6 Polyclonal antibody (Product # PA5-121253) at a dilution of 1:1,000. 25 µg of lysate or protein were loaded into each lane. A blocking buffer of 3% nonfat dry milk in TBST was used. An HRP conjugated Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a 1:10,000 dilution. Chemiluminescent detection was performed for 1s.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of FRMD6 in C6 cells using a FRMD6 Polyclonal antibody (Product # PA5-121253). Blue: DAPI for nuclear staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemical analysis of FRMD6 in C6 cells using a FRMD6 Polyclonal antibody (Product # PA5-121253). Blue: DAPI for nuclear staining.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRMD6 in human thyroid cancer using a Polyclonal antibody (Product # PA5-121253) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRMD6 in human thyroid cancer using a Polyclonal antibody (Product # PA5-121253) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of FRMD6 in human thyroid cancer using a Polyclonal antibody (Product # PA5-121253) at a dilution of 1:100.
